Die Beautiful follows the life and death of transgender woman Trisha, who aspires to be a beauty queen. Through a nonlinear timeline, the film explores Trisha's relationships, struggles with rejection and transphobia, and her journey towards self-acceptance. The story touches upon themes of family, friendship, and the pursuit of one's dreams.

After her best friend dies, Barbs starts a new life as a straight man named Bobby, which leads her to Trisha's ex-boyfriend Michaelangelo, to her own ex-boyfriend Greg and to a woman claiming she is pregnant with Barbs' child.