Miles Morales is now a fugitive on the run from every other Spider in the multiverse, and Gwen and his other friends might not be enough to help him.

When teenager Miles Morales gets bitten by a radioactive spider, he becomes the new Spider-Man in his dimension. However, he soon discovers that there are other versions of Spider-Man from alternate dimensions. Miles must team up with these different versions, including Peter Parker, Gwen Stacy, Spider-Man Noir, Spider-Ham, and Peni Parker, to stop the villainous Kingpin from opening portals to other dimensions and destroying New York City. Along the way, Miles learns what it truly means to be a hero.

Miles Morales, along with a team of Spider-People, must protect the multiverse when they clash on how to handle a new threat, redefining what it means to be a hero.