A beautiful school is about to reopen after many years and Luì and Sofì (Me against You) are the special guests of the inauguration party, their friend Pongo awaits them there. The school, however, could hide a mystery and, once again, the Me against You will have to face the evil Mr. S with courage and try to sabotage his evil plans in the name of friendship. In the school, for the first time, Luì and Sofì could learn an important secret about their past. A new “magical” adventure in the cinema for Luì and Sofì, in a world of magic, with lots of surprises and fun for their little fans and all families.

A young couple who makes popular YouTube videos for children sets out to win an award, but an evil mastermind stands in the way of their success.

The alliance of Malefics, formed by Mr. S., Perfidia, Viperiana and Serpe have convinced the world that He and Sophi are criminals and the two, in order not to be wanted, join other characters to prove their innocence and bring harmony back to the planet.

After their adventures in Ancient Egypt, Luì and Sofì just want to rest, but a new threat looms over the Earth. A magical spring hidden in the jungle holds the key to undoing all the world's spells, but if tainted, it can turn the Earth into a barren, lifeless wasteland. Viperiana has discovered this and with the help of her faithful Serpe servant has already started mixing enchanted potions in the spring that turn the water purple; it will only take a few days for everything to be irreversibly poisoned. A fight against time begins for Luì and Sofì who, together with Pongo's assistant and accompanied by the guide Serenino and Tara, daughter of the Pesantosi tribal chief, will have to decipher the map that will lead them to the magical spring and foil Viperiana's diabolical plan . But they have to be careful, because an unexpected enemy lurks among them.

Luì is about to receive his diploma as a scientist until Mr. S and Perfidia will try to undermine the Me against You.